Choline chloride content testing methods commonly used in the industry.
Molecular Formula: HOCH 2 CH 2 N+(CH 3 ) 3 CL
Molecular Weight: 139.631. Non-aqueous titration method
Weigh accurately about 0.7g(to be accurate up to 0.0002g) of the sample previously dried at 80 for 3 hours, put it into 250ml Erlenmeyer flask and add 40ml of methanol. Swirl the Erlenmeyer flask adequately and then filter after 30 minutes. Wash the precipitate with 20,15,and 15ml of methanol respectively for 3 times. Incorporate the filtrate and filtrate and dry it on the bath for spare. Add 20ml of ice Acetate to the above-mentioned Erlenmeyer flask and dissolve. Then add 2ml of Acetic Anhydride, 10ml of Hg Acetate solution and two drops crystal violet index liquid, and shake it. Titrate it with standard perchloric acid titration solution until it becomes pure blue and meanwhile carry out blank test.
Measure the content of Choline Chloride X:
X=C ¡Á (V-V 0 ) ¡Á 139.63 /(m ¡Á 1000)
C-- Actual concentration of standard perchloric acid titration solution,mol/L.
V-- size of perchloric acid's standard titration solution in the case of samples consumptin,ml.
V 0 -- size of perchloric acid's standard titration solution in the case of blank consumption,ml.
M-- the weight of samples,g.
139.63-- choline chloride's mol quality £¬ g/mol ¡£
2. Chloric Ion Testing Method
Weight 0.5g sample, put in 250ml Iodic Measuring flask, add 50ml water and shake until equable, add 0.5ml Kalium Chromic Acid (K 2 CrO 4 ) £¬ Titrate Silver Nitrate(AgNO 3 ) standard solution nitration to brick red, simultaneously do the blank text.
(1)Content of Choline Chloride = C ¡Á£¨ V-V ¡££©¡Á 139.63 £© / £¨ m ¡Á 1000 £©
(2)Content of Chlorin= C ¡Á£¨ V-V ¡££©¡Á 35.50 £© / £¨ m ¡Á 1000 £©
50% Choline Chloride it have 12.71% Chlorin in
60% Choline Chloride it have 15.25% Chlorin in
C ¡ª¡ª Silver Nitrate standard solution's concentration £¬ mol/L £»
V ¡ª¡ª Silver Nitrate titration quantum £¬ ml £»
V ¡£¡ª¡ª blank titration quantum £¬ ml £»
139.63 ¡ª¡ª choline chloride's mol quality £¬ g/mol ¡£
3. Sodium Tetraphenylborate Testing Method
Weight about 1g sample(exact weight is 0.0002g), put in 100ml capacity flask, add water to resolve and dilute until graduation, shake equally, filter, exactly take 10ml to put in 100ml beaker, add 20ml water, one drop Aluminum Oxide (10%),20ml Sodium Tetraphenylborate(2%, pure Sodium Tetraphenylborate 2g in good class, add water to resolve and dilute until 100ml, add two drops of 0.5mol/L NaOH, put in 24h after shake equally, filter, expiry date of this solution is 5d), continuously shake 30 minutes, filter with weight no. 4 crucible which is dried by heat in advance, wash beaker with water four times, 10ml every time, and filter filtrate together with, sludge and dry the sediments by heat under 105 ¡æ for 2 hours , take it out and put in desiccator for cool off, then weight.
Content of Choline Chloride = (the weight of sediments £¨ g £©¡Á 0.3298 ¡Á 100)/ £¨ weight of sample £¨ g £©¡Á 10 £©
0.3298 ¡ª¡ª 1 g weight of sediments is 0.3298 g weight of Choline Chloride
4. Nitrogen Ion Testing Method
£¨ 1 £© . Silica carrier
Weight 0.5g, 0.6g sample use Nitrogen Ion Testing Method to mensurate content of nitrogen
Content of Choline Chloride = mensurative Choline Chloride's Nitrogen content/pure Choline Chloride content(10.03) ¡Á 100%
£¨ 2 £© . Corn Cob carrier
Weight 1g sample, put in funnel with white nature determined filter paper £¬ overwash ten times with water, take filter paper and dry by heat, use Nitrogen Ion Testing Method to mensurate content of Nitrogen (N1), simultaneously weight 0.5g-0.6g and directly measure content of Nitrogen (N2).
Content of Choline Chloride = £¨ N2-N1 £© /10.03 ¡Á 100%
N2 ¡ª¡ª Directly tested content of Nitrogen
N1 ¡ª¡ª Residue(carrier)'s Nitrogen content £»
10.03 ¡ª¡ª Nitrogen's content of pure Choline Chloride ¡£
5. Chloric Ion Testing Method
Weight 0.5g sample, put in 250ml Iodic Measuring flask, add 50ml water and shake until equable, add 0.5ml Kalium Chromic Acid (K 2 CrO 4 ) £¬ Titrate Silver Nitrate(AgNO 3 ) standard solution nitration to bricky red, simultaneously make blank text.
Content of Choline Chloride = C ¡Á£¨ V-V ¡££©¡Á 35.5 £© / £¨ m ¡Á 1000 £©
Percentage for the Chloric Ion of Choline Chloride 50% is 12.71%
Percentage for the Chloric Ion of Choline Chloride 60% is 12.71%
C ¡ª¡ª Silver Nitrate standard solution's concentration £¬ mol/L £»
V ¡ª¡ª Silver Nitrate titration quantum £¬ ml £»
V ¡£¡ª¡ª blank titration quantum £¬ ml £»
35.5 ¡ª¡ª Chloric Ion's mol quality £¬ g/mol ¡£
6. PHLC Testing Method
